"In Japanese, Birdo is said to be a male who believes she is a female and prefers to be called by a nickname, "Cathie", rather than her real name "Catherine"."
"In the game Captain Rainbow, Birdo is heard with a deep, male voice which gets high-pitched when she gets angry or excited."
"Nintendo of America usually refers to Birdo as female. The femininity of Birdo is implied in Mario Kart 8, where she appears on "Women of Racing Organization" advertisements in N64 Royal Raceway along with other female characters, and in Sunshine Airport, where she is referred to on the broadcasting system as "Ms. Birdo"."
"Paper Mario: Sticker Star
“Heart of a woman, heart of a man... Both can know of love's grace...”"
"In the Japan-only game Captain Rainbow, Birdo is a damsel-in-distress. She lives on Mimin Island, in the so-called Kawaii Ouchi, or Cute Home.
In the game, Birdo is imprisoned for using the female bathroom, as the robot jailer does not believe her when she insists she is a girl.
She gets quite upset about this, and asks Nick to go to her Cute Home and find something that will prove she is not lying.
Nick finds a mysterious object under Birdo's pillow (which is blurred out, though it is implied to be a vibrator), it serves its purpose and Birdo is released."
"Birdo is very flirtatious and affectionate with others, even when she does not know them very well, as evidenced in Fortune Street where her dialogue is very playful, flirty and endearing.
This is also seen in Birdo's relationship with Popple in Mario & Luigi: Superstar Saga where she expresses her high opinion of love and romance.
--after discovering that Popple did not actually love her, she knocked him right through a wall, indicating she has super-strength, as well in Mario Superstar Baseball and Mario Super Sluggers where her strongest stat is batting.
( reference to trannies being unusually strong in competition when pretending to be women )"
"In Super Smash Bros. Brawl, Birdo's trophy description describes the character as "gender indeterminate", and uses the pronoun "it" rather than gender-specific pronouns such as "he" or "she" (in contrast to Super Smash Bros."
""Oh! But I forgot to introduce myself. I'm Birdo, the camp-house mama. You can even CALL me "mama" if you want!" - Mario Golf: Super Rush"
In Mario Party, Birdo grunts and snorts in nasal male tones.
Captain Rainbow, a COUNTRY RESTRICTED homosexual nintendo game.

article about how captain rainbow finds birdo's vibrator:
https://www.gamezone.com/originals/captain-rainbow-finds-disturbing-proof-birdo-is-a-woman/