No, because I've seen Mr Beast keep referring to Chris as "he".
He doesn't believe in the whole trans crap, but he plays along because he doesn't want to suffer the backlash of not playing along.
And I'm 100% sure that Chris knows this. I believe he trooned out to make a "power grab". He was always the sidekick in Mr Beast videos, but now he holds the reins. All the attention is on him. And he can force Mr Beast, and everyone else he works with, to conform to his new reality. He trooned out to gain attention and power he felt he was lacking. Now everyone has to bow to his will, or else.