This clip is pretty damning, especially alongside the context of the 10 hour datestream.
As much as Nick's defenders have said that it's normal for hetero dudes to act ironically gay, and joke around about gay stuff, it's even more normal for them to talk about girls, or want to go somewhere girls will be to meet some, or at least check them out, when they're hanging out together for 10 straight hours, especially when they're at the age Nick and Catboy are at.
Catboy finding it so hilarious when someone asks if Nick likes girls, and then following it up with his serious answer of the generic "he wants a wife and family someday", which is basically a Fuentes tradcatholic talking point, is about the worst way he could've handled that question short of a catty, jealous tirade