Well, one of them was a troon.
To be fair, the only major character I can think of that's mentioned to be a lesbian in dialogue in the game itself was the villain and some of her coven. Which is a lot of characters relative to the minimal cast, but I didn't care too much because I spent that mission choking all of them out, and used Far Reach to throw Delilah off of a cliff.
Granted when you look into the notes and journals you find out that pretty much every woman is into women. Emily has a love letter that never specifies her lover's sex (even though the name is basically the word woman but y instead of the o), but I think I remember someone from the studio said Emily was supposed to be bi which is why they didn't specify. Billy Lurk has a letter from a female ex. Hell, Aramis Stilton is also gay, though that was more implied than the others being outright stated. For how small the cast of major characters is, the percentage that are some flavor of LGBT is insane.