Are you getting the vaccine? - Absolute trashfire thread, please enter with caution

Can someone spoonfeed me on how they get the mRNA? The official CDC website says the mRNA in the vaccines is made "in a laboratory". What the fuck does that mean? Are they 3D printed with goo?
 
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Null said:
Can someone spoonfeed me on how they get the mRNA? The official CDC website says the mRNA in the vaccines is made "in a laboratory". What the fuck does that mean? Are they 3D printed with goo?
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They get it from aliens and then add in the 5G nanoparticles so they can turn on the zombie virus in whoever got the retard juice, or so I hear. Since I got the retard juice, if I ever start talking sense, that's probably when they turned it on.
 
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Imagine missing the chance to become absolutely gigavaxxed:
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According to the scientists, certain immune cells and antibodies against Sars-CoV-2 are significantly more common in him than in people who only received three vaccinations. Until now, it was unclear what effects so-called hypervaccination has on the immune system.
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You all had your chance; good thing the vaccines you refused could instead be used by vaxxmaxxers.
 
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Null said:
Can someone spoonfeed me on how they get the mRNA? The official CDC website says the mRNA in the vaccines is made "in a laboratory". What the fuck does that mean? Are they 3D printed with goo?
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Step 1 is they purify viral RNA straight from a sample of the virus.

Step 2 is they reverse transcribe that pure RNA into complementary DNA with a protein called reverse transcriptase. DNA stocks for RNA production are preferred over straight RNA templates since DNA is more chemically stable and easier to reproduce (either synthetically or through a model organism).

Step 3 is to then synthesize mRNA with the DNA as a template in a process called transcription. This RNA polymerase protein is specifically made to read DNA templates and produce the RNA sequence for them. This RNA is then converted into mRNA with a few other chemical modifications afterwards on both ends of the sequence to stop it from being digested in the cell.

Here a pic summarizing this reaction: IMG_0659.jpeg

I’m not sure what tricks go into running this sort of production on an industrial level efficiently but those are the basic principles behind RNA production.
 
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Null said:
Can someone spoonfeed me on how they get the mRNA? The official CDC website says the mRNA in the vaccines is made "in a laboratory". What the fuck does that mean? Are they 3D printed with goo?
Click to expand...
Colloid said:
Step 1 is they purify viral RNA straight from a sample of the virus.

Step 2 is they reverse transcribe that pure RNA into complementary DNA with a protein called reverse transcriptase. DNA stocks for RNA production are preferred over straight RNA templates since DNA is more chemically stable and easier to reproduce (either synthetically or through a model organism).

Step 3 is to then synthesize mRNA with the DNA as a template in a process called transcription. This RNA polymerase protein is specifically made to read DNA templates and produce the RNA sequence for them. This RNA is then converted into mRNA with a few other chemical modifications afterwards on both ends of the sequence to stop it from being digested in the cell.

Here a pic summarizing this reaction: View attachment 5789371

I’m not sure what tricks go into running this sort of production on an industrial level efficiently but those are the basic principles behind RNA production.
Click to expand...
Just to add, the technique is called In Vitro Transcription. Basically get a DNA and throw a bunch of chemicals on top, leave for a while and the chemical reaction will produce mRNA.
 
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Null said:
are all vaccines made from fetal tissue or something?
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My Nullga WHAT?

Maybe there is some technique that uses that but i am not aware. But there are several ways to get a vaccine, from cultivating them in a host, like chicken eggs, until they get weaker but retain their markers (so the immune system can sill recognize them), to even shredding them and leaving just pieces with enough markers. mRNA is the new one.

Maybe if you are referring to fetal tissues from chicken eggs, the chicken embryos, sure. But it implies fetal tissue from human babies, maybe in the US with planned parenthood. but not anywhere else in the world.
 
No, they used aborted fetal tissue for the development of vaccines. That's not a question. My question is if they use it for all industrial production.
 
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Null said:
No, they used aborted fetal tissue for the development of vaccines. That's not a question. My question is if they use it for all industrial production.
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Yeah, sure for the development. They also use for the development of artificial sweeteners since the 70's if i remember correctly. But in large scale production it doesn't make a lot of sense. I can't confirm to you in the US, but where i live the chicken egg technique is used for the flu vaccine.

EDIT: I think you are referring to the use of the HEK 293 cells during development ( https://en.wikipedia.org/wiki/HEK_293_cells ). Immortal culture cells from a aborted baby's kidney in the 70's.
 
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Null said:
No, they used aborted fetal tissue for the development of vaccines. That's not a question. My question is if they use it for all industrial production.
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Yes, used pretty much across the board during development, and some like the J&J covid vaccine mass produce it using fetal cells.

The mRNA vaccines use non fetal cells in their bioreactors (or at least moderna does).
 
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