I doubt a troon saga would ever be in the works for the Bob Show. Kino Roberto is, at his core, very much a toxic white cis male, and he is way too caught up in his own self-image of being the pinnacle of non-obsolete masculinity. At most he'll pay lip service to some general "mine and basically everyone's gender and orientation is kinda fluid, it's 2022, friendo" kind of sentiment.
I also don't think he'd an hero if his ego gets shot up enough for him to realize what a pathetic waste of space he is.
Mostly, I see one of two things happening:
1) He realizes that he's done for, and will try to be the bigger man (well, he's always the bigger man because he's fat) and "graciously" leave the public eye. No idea what he'd do instead, but he'd bow out of Twitter at least for a while and try to weather it out. He'll self-flagellate a bit and hang his head in shame and use the ordeal to do even more posturing.
2) If things get really rough and there's absolutely nobody left to support him, he might swallow the black pill, dig in, and go Full tismSpectrum Warrior solo commando against everything and everyone. It'd really be a complete descent into madness with some proper schizoposting mixed in. He's barely coherent right now, but such a blow might push him over the edge. He'd probably develop more medical issues quickly after that, and either fade away from public view due to hospitalizations, or just die.
The first option seems more in line with him, even though the very first sentence says "He realizes", which is not something Bob does, ever. Still, it's the more likely option for when there's still a modicum of support for him around. If he still has a few troon stans left, he'll grovel and self-flagellate like a good boi.
Second option is unlikely, but if he gets hit with a barrage of blows in quick succession (Trump reelection, metoo'd, ostracized by all his favourite waifus in public, denounced by his troons, an AI trained with his reviews and tweets replaces him) his mind might break and he could see himself turning more into an anti-hero. Remember, Bob thinks in terms of movies and their internal rules. He either has to redeem himself, or go fight as the lone wolf for justice.
/edit:
Oh yeah, if he actually gets busted for something REALLY, criminally bad, like child porn or worse, he might actually an hero. But not for a mild case Metoo because he got drunk with a bimbo at a con or shit like that.